Open AccessPatterned deposition of cells and proteins onto surfaces by using three-dimensional microfluidic systems
Author(s) -
Daniel T. Chiu,
Noo Li Jeon,
Sui Huang,
Ravi S. Kane,
Christopher J. Wargo,
Insung S. Choi,
Donald E. Ingber,
George M. Whitesides
Publication year - 2000
Publication title -
proceedings of the national academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.040562297
Subject(s) - microfluidics , substrate (aquarium) , nanotechnology , planar , materials science , channel (broadcasting) , deposition (geology) , layer (electronics) , topology (electrical circuits) , biophysics , chemistry , biology , computer science , engineering , ecology , computer network , paleontology , computer graphics (images) , sediment , electrical engineering
Three-dimensional microfluidic systems were fabricated and used to pattern proteins and mammalian cells on a planar substrate. The three-dimensional topology of the microfluidic network in the stamp makes this technique a versatile one with which to pattern multiple types of proteins and cells in complex, discontinuous structures on a surface. The channel structure, formed by the stamp when it is in contact with the surface of the substrate, limits migration and growth of cells in the channels. With the channel structure in contact with the surface, the cells stop dividing once they form a confluent layer. Removal of the stamp permits the cells to spread and divide.
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