Open AccessA Populus EST resource for plant functional genomics
Author(s) -
Fredrik Sterky,
Rupali Bhalerao,
Per Unneberg,
Bo Segerman,
Peter Nilsson,
Amy M. Brunner,
Laurence Charbonnel-Campaa,
Jenny Jonsson Lindvall,
Karolina Tandre,
Steven H. Strauss,
Björn Sundberg,
Petter Gustafsson,
Mathias Uhlén,
Rishikesh P. Bhalerao,
Ove Nilsson,
Göran Sandberg,
Jan Karlsson,
Joakim Lundeberg,
Stefan Jansson
Publication year - 2004
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.0401641101
Subject(s) - arabidopsis , functional genomics , biology , genome , genomics , computational biology , function (biology) , expressed sequence tag , gene , evolutionary biology , genetics , mutant
Trees present a life form of paramount importance for terrestrial ecosystems and human societies because of their ecological structure and physiological function and provision of energy and industrial materials. The genus Populus is the internationally accepted model for molecular tree biology. We have analyzed 102,019 Populus ESTs that clustered into 11,885 clusters and 12,759 singletons. We also provide >4,000 assembled full clone sequences to serve as a basis for the upcoming annotation of the Populus genome sequence. A public web-based EST database (POPULUSDB) provides digital expression profiles for 18 tissues that comprise the majority of differentiated organs. The coding content of Populus and Arabidopsis genomes shows very high similarity, indicating that differences between these annual and perennial angiosperm life forms result primarily from differences in gene regulation. The high similarity between Populus and Arabidopsis will allow studies of Populus to directly benefit from the detailed functional genomic information generated for Arabidopsis, enabling detailed insights into tree development and adaptation. These data will also valuable for functional genomic efforts in Arabidopsis.