Tracking an embryonic L1 retrotransposition event | Zendy

Eline T. Luning Prak | Zendy; Allen W. Dodson | Zendy; Evan A. Farkash | Zendy; Haig H. Kazazian | Zendy

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Tracking an embryonic L1 retrotransposition event

Author(s) -

Eline T. Luning Prak,

Allen W. Dodson,

Evan A. Farkash,

Haig H. Kazazian

Publication year - 2003

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.0337627100

Subject(s) - retrotransposon , biology , green fluorescent protein , gene , transcription (linguistics) , transgene , reverse transcriptase , genetics , microbiology and biotechnology , rna , genome , transposable element , linguistics , philosophy

Long interspersed nuclear elements 1 (L1) are active retrotransposons that reside in many species, including humans and rodents. L1 elements produce an RNA intermediate that is reverse transcribed to DNA and inserted in a new genomic location. We have tagged an active human L1 element (L1(RP)) with a gene encoding enhanced GFP (EGFP). Expression of GFP occurs only if L1-EGFP has undergone a cycle of transcription, reverse transcription, and integration into a transcriptionally permissive genomic region. We show here that L1-EGFP can undergo retrotransposition in vivo and produce fluorescence in mouse testis. The retrotransposition event characterized here has occurred at a very early stage in the development of an L1-EGFP transgenic founder mouse.

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