Open AccessModification of the erythroid transcription factor GATA-1 by SUMO-1
Author(s) -
Licio Collavin,
Monica Gostissa,
Fabio Avolio,
Paola Secco,
Antonella Ronchi,
Claudio Santoro,
Giannino Del Sal
Publication year - 2004
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.0308605101
Subject(s) - sumo protein , transcription factor , microbiology and biotechnology , gata transcription factor , biology , xenopus , transcription (linguistics) , mutant , ets transcription factor family , general transcription factor , gata2 , promoter , ubiquitin , gene expression , gene , biochemistry , linguistics , philosophy
The activity of transcription factors is tightly modulated by posttranslational modifications affecting stability, localization, and protein-protein interactions. Conjugation to SUMO is a reversible posttranslational modification that has been shown to regulate important transcription factors involved in cell proliferation, differentiation, and tumor suppression. Here, we demonstrate that the erythroid transcription factor GATA-1 is sumoylated in vitro and in vivo and map the single lysine residue involved in SUMO-1 attachment. We show that the nuclear RING finger protein PIASy promotes sumoylation of GATA-1 and dramatically represses its transcriptional activity. We present evidence that a nonsumoylatable GATA-1 mutant is indistinguishable from the WT protein in its ability to transactivate a reporter gene in mammalian cells and in its ability to trigger endogenous globin expression in Xenopus explants. These observations open interesting questions about the biological role of this posttranslational modification of GATA-1.