The accuracy of codon recognition by polypeptide release factors | Zendy

David V. Freistroffer | Zendy; Marek Kwiatkowski | Zendy; Richard H. Buckingham | Zendy; Måns Ehrenberg | Zendy

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The accuracy of codon recognition by polypeptide release factors

Author(s) -

David V. Freistroffer,

Marek Kwiatkowski,

Richard H. Buckingham,

Måns Ehrenberg

Publication year - 2000

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.030541097

Subject(s) - release factor , transfer rna , proofreading , stop codon , ribosome , genetics , biology , codon usage bias , translation (biology) , sense (electronics) , reading frame , protein biosynthesis , gene , open reading frame , messenger rna , chemistry , peptide sequence , polymerase , rna , genome

The precision with which individual termination codons in mRNA are recognized by protein release factors (RFs) has been measured and compared with the decoding of sense codons by tRNA. An Escherichia coli system for protein synthesis in vitro with purified components was used to study the accuracy of termination by RF1 and RF2 in the presence or absence of RF3. The efficiency of factor-dependent termination at all sense codons differing from any of the three stop codons by a single mutation was measured and compared with the efficiency of termination at the three stop codons. RF1 and RF2 discriminate against sense codons related to stop codons by between 3 and more than 6 orders of magnitude. This high level of accuracy is obtained without energy-driven error correction (proofreading), in contrast to codon-dependent aminoacyl-tRNA recognition by ribosomes. Two codons, UAU and UGG, stand out as hotspots for RF-dependent premature termination.

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