Open AccessThe Uptake of Sucrose by Bean Leaf Tissue II. Kinetic Experiments
Author(s) -
RS Vickery,
FV Mercer
Publication year - 1967
Publication title -
australian journal of biological sciences
Language(s) - English
Resource type - Journals
ISSN - 0004-9417
DOI - 10.1071/bi9670565
Subject(s) - sucrose , volume (thermodynamics) , osmotic pressure , kinetics , concentration gradient , phaseolus , osmosis , chemistry , osmotic shock , biochemistry , biophysics , botany , biology , chromatography , thermodynamics , membrane , physics , quantum mechanics , gene
Leaf tissue of Phaseolus vulgari8 takes up sucrose from external solutions by two processes. The first is an osmotic uptake into the free space. The second is a non-osmotic uptake into the osmotic volume. The kinetics of the non-osmotic uptake, from solutions less concentrated than 0 lM, fit the Michaelis-Menton equation. The tissue can accumulate sucrose against its concentration gradient from solutions less concentrated than 0 lM. There is no significant efflux of sucrose from the tissue. The correlation of the rate of carbon dioxide droduction with the internal sucrose concentration suggests that part, at least, of the cytoplasm is included in the osmotic volume for sucrose.