Rotational manipulation of a microscopic object inside a microfluidic channel

Observations and analyses of a microscopic object are essential processes in various fields such as chemical engineering and life science. Microfluidic techniques with various functions and extensions have often been used for such purposes to investigate the mechanical properties of microscopic objects such as biological cells. One of such extensions proposed in this context is a real-time visual feedback manipulation system, which is composed of a high-speed camera and a piezoelectric actuator with a single-line microfluidic channel. Although the on-chip manipulation system enables us to control the 1 degree-of-freedom position of a target object by the real-time pressure control, it has suffered from unintended changes in the object orientation, which is out of control in the previous system. In this study, we propose and demonstrate a novel shear-flow-based mechanism for the control of the orientation of a target object in addition to the position control in a microchannel to overcome the problem of the unintended rotation. We designed a tributary channel using a three-dimensional hydrodynamic simulation with boundary conditions appropriate for the particle manipulation to apply shear stress to the target particle placed at the junction and succeeded in rotating the particle at an angular velocity of 0.2 rad/s even under the position control in the experiment. The proposed mechanism would be applied to feedback controls of a target object in a microchannel to be in a desired orientation and at a desired position, which could be a universally useful function for various microfluidic platforms.