Fluorescence strategies for mapping cell membrane dynamics and structures | Zendy

Jagadish Sankaran | Zendy; Thorsten Wohland | Zendy

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Fluorescence strategies for mapping cell membrane dynamics and structures

Author(s) -

Jagadish Sankaran,

Thorsten Wohland

Publication year - 2020

Publication title -

apl bioengineering

Language(s) - English

Resource type - Journals

ISSN - 2473-2877

DOI - 10.1063/1.5143945

Subject(s) - membrane , fluorescence spectroscopy , microsecond , fluorescence , nanotechnology , spectroscopy , membrane biophysics , chemistry , fluorescence correlation spectroscopy , biophysics , materials science , biological membrane , physics , biology , optics , biochemistry , quantum mechanics

Fluorescence spectroscopy has been a cornerstone of research in membrane dynamics and organization. Technological advances in fluorescence spectroscopy went hand in hand with discovery of various physicochemical properties of membranes at nanometric spatial and microsecond timescales. In this perspective, we discuss the various challenges associated with quantification of physicochemical properties of membranes and how various modes of fluorescence spectroscopy have overcome these challenges to shed light on the structure and organization of membranes. Finally, we discuss newer measurement strategies and data analysis tools to investigate the structure, dynamics, and organization of membranes.

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