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The function of ruminant feed supplements is to fulfil nutritional requirements to optimize rumen performance. Rumen microbes work optimally if the nutrient sources in the feed are always available and easily degraded. The source of the nutrients can be obtained from agricultural products, plantation products, agricultural industries and food [1]. Tanin and saponins from the plant are defaunation agents that are widely used in several studies. Tanin can be used as a defaunation agent which can reduce the protozoa population and suppress methane emissions in the rumen. Observations were using Randomized Block Design of 4 treatments and 4 groups with 3 replications in each group. The treatments observed were as follows: R0: base ration without extract (control), R1: base ration + 2% extract of cassava leaves, R2: base ration + 2% extract of Indigofera zollingeriana leaves, R3: base ration + 1% extract of cassava leaves + 1% extract of Indigofera zollingeriana leaves. The results showed that treatments of R3 significantly effective for fermentation system of in vitro by measurement at the degradation of dry matter and organic matter also at total gas production and methane production.The function of ruminant feed supplements is to fulfil nutritional requirements to optimize rumen performance. Rumen microbes work optimally if the nutrient sources in the feed are always available and easily degraded. The source of the nutrients can be obtained from agricultural products, plantation products, agricultural industries and food [1]. Tanin and saponins from the plant are defaunation agents that are widely used in several studies. Tanin can be used as a defaunation agent which can reduce the protozoa population and suppress methane emissions in the rumen. Observations were using Randomized Block Design of 4 treatments and 4 groups with 3 replications in each group. The treatments observed were as follows: R0: base ration without extract (control), R1: base ration + 2% extract of cassava leaves, R2: base ration + 2% extract of Indigofera zollingeriana leaves, R3: base ration + 1% extract of cassava leaves + 1% extract of Indigofera zollingeriana leaves. The results showed that treatments of R3...

Supplementation effectivity of cassava and Indigofera zollingeriana leaves extraction on rumen fermentation system of in vitro