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Enantiomers can have different pharmacological effects. Therefore, it is necessary to be able to separate pure enantiomers that have a beneficial biological activity. The purpose of this study was to modify a monolith column in stationary phase with Human Serum Albumin (HSA) for use in separating chiral compounds by HPLC. In this work, poly(glycidyl methacrylate-co-ethylene glycol dimethacrylate) (poly(GMA-co-EDMA)) and poly(glycidyl methacrylate-co-trimethylolpropane trimethacrylate) (poly(GMA-co-TRIM)) columns were in-situ co-polymerized inside a silicosteel tubing. A total monomer ratio (% T) and crosslinking agent (% C) 40:25 and 28:12 were applied for the preparation of poly(GMA-co-EDMA) and poly(GMA-co-TRIM), respectively. The porogen used was 1-propanol, 1,4-butanediol, and water in a ratio of 7:4:1 (v/v) and AIBN as an initiator (1% of the total monomer). The column was polymerized at 60 °C for 12 h, then immobilized with various concentrations of Human Serum Albumin (HSA). The monolithic poly(GMA-co-TRIM) column modified with HSA 1 mg/ml was successfully applied to separation of the enantiomers of citronellal using an acetonitrile and water (50:50, v/v) as the mobile phase at 0.05 ml/min. The permeability of this optimized monolithic column was 0.0018 Darcy.Enantiomers can have different pharmacological effects. Therefore, it is necessary to be able to separate pure enantiomers that have a beneficial biological activity. The purpose of this study was to modify a monolith column in stationary phase with Human Serum Albumin (HSA) for use in separating chiral compounds by HPLC. In this work, poly(glycidyl methacrylate-co-ethylene glycol dimethacrylate) (poly(GMA-co-EDMA)) and poly(glycidyl methacrylate-co-trimethylolpropane trimethacrylate) (poly(GMA-co-TRIM)) columns were in-situ co-polymerized inside a silicosteel tubing. A total monomer ratio (% T) and crosslinking agent (% C) 40:25 and 28:12 were applied for the preparation of poly(GMA-co-EDMA) and poly(GMA-co-TRIM), respectively. The porogen used was 1-propanol, 1,4-butanediol, and water in a ratio of 7:4:1 (v/v) and AIBN as an initiator (1% of the total monomer). The column was polymerized at 60 °C for 12 h, then immobilized with various concentrations of Human Serum Albumin (HSA). The monolithic poly(GMA...

Modification of monolithic stationary phase using human serum albumin as chiral separation