Combination effect of Centella asiatica (L.) urban and Pluchea indica (L.) urban on uterus weight and uterus and oviduct histological profiles of Rattus norwegicus

The use of herbs as a medicinal material is currently increasing because they are considered to have lower negative effects than synthetic drugs. Centella asiatica and Pluchea indica allegedly contain compounds that can affect the female reproductive organs, such as the uterus and oviduct, and reproductive hormone levels. The main function of the uterus is as a place of implantation and the oviduct as a place of fertilization, so that if there is interference in either of these organs reproductive function will also be disrupted. This study used completely randomized design with six treatments and four replicates. The treatment used was a combination of Centella asiatica and Pluchea indica at dosages of 0 mg/kg bw (control), 25 mg/kg bw, 50 mg/kg bw, 75 mg/kg bw, 125 mg/kg bw and 200 mg/kg bw for 18 days. Estrus synchronization was performed by prostaglandin (Lutalyse) injection 3 days before treatment. The parameters observed were uterine weight, endometrial thickness, myometrial thickness, number of endometrial glands, oviduct mucosal thickness, oviduct smooth muscle thickness and estrogen and progesterone levels. The uterus and oviduct were prepared with hematoxylin and eosin staining. Data were analyzed by ANOVA and with LSD test. The results showed that the combination of leaf extracts of Centella asiatica and Pluchea indica had an effect on the histological profile of uterus and oviduct and estrogen and progesteron levels, but did not have an effect on the weight of uterus.