Open AccessLasing at 602-620 nm from a red algae-derived phycobiliprotein
Author(s) -
José A. Rivera,
J. G. Eden
Publication year - 2017
Publication title -
apl photonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.094
H-Index - 34
ISSN - 2378-0967
DOI - 10.1063/1.4999716
Subject(s) - phycobiliprotein , laser , lasing threshold , photoexcitation , materials science , optics , full width at half maximum , fluorescence , fluence , phycoerythrin , optoelectronics , allophycocyanin , analytical chemistry (journal) , chemistry , excited state , atomic physics , physics , biology , chromatography , cyanobacteria , flow cytometry , genetics , phycocyanin , bacteria
Lasing in the 602-620 nm (orange-red) spectral region has been observed from R-phycoerythrin (RPE), a phycobiliprotein responsible for the >80% quantum yield of red algae. Photoexcitation of 8 μM RPE solutions in a Fabry-Pérot resonator with frequency-doubled Nd:YAG laser pulses (λp = 532 nm) yields >26 nJ of energy in ∼2.5 ns (FWHM) pulses, which corresponds to >10 W of peak power. Maximum laser emission occurs at ∼610 nm, the threshold pump energy fluence is measured to be 260 ± 15 μJ/mm2, and more than 100 longitudinal laser modes are generated when the cavity free spectral range and mirror separation are Δλ = 0.18 nm and L = 777 ± 1 μm, respectively. In combination with the known versatility of the phycobiliproteins as fluorescent tags, the biomolecular RPE laser reported here suggests its applicability to clinical assays and in situ laser imaging
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