Open AccessHolographic characterization of colloidal particles in turbid media
Author(s) -
Fook Chiong Cheong,
Priya Kasimbeg,
David B. Ruffner,
Ei Hnin Hlaing,
Jaroslaw M. Blusewicz,
Laura A. Philips,
David G. Grier
Publication year - 2017
Publication title -
applied physics letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.182
H-Index - 442
eISSN - 1077-3118
pISSN - 0003-6951
DOI - 10.1063/1.4999101
Subject(s) - holography , characterization (materials science) , light scattering , microscopy , optics , materials science , soft matter , particle (ecology) , scattering , colloid , mie scattering , refractive index , dynamic light scattering , nanoparticle , nanotechnology , chemistry , physics , oceanography , geology
Holographic particle characterization uses in-line holographic microscopy and the Lorenz-Mie theory of light scattering to measure the diameter and the refractive index of individual colloidal particles in their native dispersions. This wealth of information has proved invaluable in fields as diverse as soft-matter physics, biopharmaceuticals, wastewater management, and food science but so far has been available only for dispersions in transparent media. Here, we demonstrate that holographic characterization can yield precise and accurate results even when the particles of interest are dispersed in turbid media. By elucidating how multiple light scattering contributes to image formation in holographic microscopy, we establish the range conditions under which holographic characterization can reliably probe turbid samples. We validate the technique with measurements on model colloidal spheres dispersed in commercial nanoparticle slurries.
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