Compartmentalized embryoid body culture for induction of spatially patterned differentiation | Zendy

Shohei Kaneda | Zendy; Jiro Kawada | Zendy; Hidenori Akutsu | Zendy; Justin K. Ichida | Zendy; Yoshiho Ikeuchi | Zendy; Teruo Fujii | Zendy

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Compartmentalized embryoid body culture for induction of spatially patterned differentiation

Author(s) -

Shohei Kaneda,

Jiro Kawada,

Hidenori Akutsu,

Justin K. Ichida,

Yoshiho Ikeuchi,

Teruo Fujii

Publication year - 2017

Publication title -

biomicrofluidics

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.634

H-Index - 63

ISSN - 1932-1058

DOI - 10.1063/1.4994989

Subject(s) - embryoid body , microbiology and biotechnology , cellular differentiation , compartment (ship) , induced pluripotent stem cell , embryonic stem cell , chemistry , stem cell , cell culture , biophysics , biology , biochemistry , genetics , oceanography , gene , geology

We developed a compartmentalized culture system of single embryoid bodies (EBs) utilizing a through-hole on a membrane to induce spatially patterned differentiation. An EB derived from mouse pluripotent stem cells was immobilized on the through-hole. By introducing a stem cell maintenance medium and a differentiation medium into upper and lower culture compartments, respectively, a localized differentiated state was achieved only in the lower part of EB, which is exposed to the medium in the lower compartment. This system may enable us to reconstruct complex tissues and to recapitulate developmental processes using EBs.

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