Open AccessZwitterion functionalized gold nanoclusters for multimodal near infrared fluorescence and photoacoustic imaging
Author(s) -
Danjin Shen,
Maxime Henry,
Vanessa Trouillet,
Clothilde CombyZerbino,
Franck Bertorelle,
Lucie Sancey,
Rodolphe Antoine,
JeanLuc Coll,
Véronique Josserand,
Xavier Le Guével
Publication year - 2017
Publication title -
apl materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.571
H-Index - 60
ISSN - 2166-532X
DOI - 10.1063/1.4977203
Subject(s) - nanoclusters , zwitterion , fluorescence , photoacoustic imaging in biomedicine , ligand (biochemistry) , fluorescence lifetime imaging microscopy , in vivo , detection limit , chemistry , molecular imaging , materials science , nanotechnology , molecule , optics , chromatography , biochemistry , receptor , biology , organic chemistry , physics , microbiology and biotechnology
Gold nanoclusters (Au NCs) are an emerging type of theranostic agents combining therapeutic and imaging features with reduced toxicity. Au NCs stabilized by a zwitterion ligand with a fine control of the metal core size and the ligand coverage were synthesized by wet chemistry. Intense fluorescence signal is reported for the highest ligand coverage, whereas photoacoustic signal is stronger for the largest metal core. The best Au NC candidate with an average molecular weight of 17 kDa could be detected with high sensitivity on a 2D-near-infrared imaging instrument (limit of detection (LOD) = 2.3 μM) and by photoacoustic imaging. In vitro and in vivo experiments demonstrate an efficient cell uptake in U87 cell lines, a fast renal clearance (t1/2α = 6.5 ± 1.3 min), and a good correlation between near infrared fluorescence and photoacoustic measurements to follow the early uptake of Au NCs in liver
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