Naturally occurring branched-chain polyamines induce a crosslinked meshwork structure in a giant DNA | Zendy

Akira Muramatsu | Zendy; Yuta Shimizu | Zendy; Yuko Yoshikawa | Zendy; Wakao Fukuda | Zendy; Naoki Umezawa | Zendy; Yuhei Horai | Zendy; Tsunehiko Higuchi | Zendy; Shinsuke Fujiwara | Zendy; Tadayuki Imanaka | Zendy; Kenichi Yoshikawa | Zendy

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Naturally occurring branched-chain polyamines induce a crosslinked meshwork structure in a giant DNA

Author(s) -

Akira Muramatsu,

Yuta Shimizu,

Yuko Yoshikawa,

Wakao Fukuda,

Naoki Umezawa,

Yuhei Horai,

Tsunehiko Higuchi,

Shinsuke Fujiwara,

Tadayuki Imanaka,

Kenichi Yoshikawa

Publication year - 2016

Publication title -

the journal of chemical physics

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.071

H-Index - 357

eISSN - 1089-7690

pISSN - 0021-9606

DOI - 10.1063/1.4972066

Subject(s) - dna , polyamine , chemistry , circular dichroism , biophysics , molecule , folding (dsp implementation) , chain (unit) , crystallography , biochemistry , stereochemistry , biology , organic chemistry , physics , astronomy , electrical engineering , engineering

We studied the effect of branched-chain polyamines on the folding transition of genome-sized DNA molecules in aqueous solution by the use of single-molecule observation with fluorescence microcopy. Detailed morphological features of polyamine/DNA complexes were characterized by atomic force microscopy (AFM). The AFM observations indicated that branched-chain polyamines tend to induce a characteristic change in the higher-order structure of DNA by forming bridges or crosslinks between the segments of a DNA molecule. In contrast, natural linear-chain polyamines cause a parallel alignment between DNA segments. Circular dichroism measurements revealed that branched-chain polyamines induce the A-form in the secondary structure of DNA, while linear-chain polyamines have only a minimum effect. This large difference in the effects of branched- and linear-chain polyamines is discussed in relation to the difference in the manner of binding of these polyamines to negatively charged double-stranded DNA.

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