Resolution enhancement of pump-probe microscopy with an inverse-annular spatial filter | Zendy

Takayoshi Kobayashi | Zendy; Koshi Kawasumi | Zendy; Jun Miyazaki | Zendy; Kazuaki Nakata | Zendy

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Resolution enhancement of pump-probe microscopy with an inverse-annular spatial filter

Author(s) -

Takayoshi Kobayashi,

Koshi Kawasumi,

Jun Miyazaki,

Kazuaki Nakata

Publication year - 2016

Publication title -

aip advances

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.421

H-Index - 58

ISSN - 2158-3226

DOI - 10.1063/1.4967948

Subject(s) - optics , microscopy , photothermal therapy , materials science , image resolution , microscope , resolution (logic) , polarization (electrochemistry) , filter (signal processing) , perpendicular , physics , chemistry , mathematics , computer science , artificial intelligence , computer vision , geometry

We have introduced a pupil filter, an inverse-annular pupil filter in a pump-probe photothermal microscope, which provides resolution enhancement in three dimensions. The resolution is probed to be improved in lateral and axial resolution by imaging experiment using 20 nm gold nanoparticles. The improvement in X (perpendicular to the common pump and probe polarization direction), Y (parallel to the polarization direction), and Z (axial direction) are by 15±6, 8±8, and 21±2 % from the resolution without a pupil filter. The resolution enhancement is even better than the calculation using vector field, which predicts the corresponding enhancement of 11, 8, and 6 %. The discussion is made to explain the unexpected results. We also demonstrate the photothermal imaging of thick biological samples (cells from rabbit intestine and kidney) stained with hematoxylin and eosin dye with the inverse-annular filter

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