A multidisciplinary approach to study the functional properties of neuron-like cell models constituting a living bio-hybrid system: SH-SY5Y cells adhering to PANI substrate | Zendy

Silvia Caponi | Zendy; Sara Mattana | Zendy; Maria Ricci | Zendy; Krizia Sagini | Zendy; León J. Juárez-Hernández | Zendy; Aura M. Jiménez-Garduño | Zendy; Nicola Cornella | Zendy; Laura Pasquardini | Zendy; Lorena Urbanelli | Zendy; Paola Sassi | Zendy; Assunta Morresi | Zendy; Carla Emiliani | Zendy; D. Fioretto | Zendy; Mauro Dalla Serra | Zendy; Cecilia Pederzolli | Zendy; Salvatore Iannotta | Zendy; Paolo Macchi | Zendy; Carlo Musio | Zendy

Open Access

A multidisciplinary approach to study the functional properties of neuron-like cell models constituting a living bio-hybrid system: SH-SY5Y cells adhering to PANI substrate

Author(s) -

Silvia Caponi,

Sara Mattana,

Maria Ricci,

Krizia Sagini,

León J. Juárez-Hernández,

Aura M. Jiménez-Garduño,

Nicola Cornella,

Laura Pasquardini,

Lorena Urbanelli,

Paola Sassi,

Assunta Morresi,

Carla Emiliani,

D. Fioretto,

Mauro Dalla Serra,

Cecilia Pederzolli,

Salvatore Iannotta,

Paolo Macchi,

Carlo Musio

Publication year - 2016

Publication title -

aip advances

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.421

H-Index - 58

ISSN - 2158-3226

DOI - 10.1063/1.4966587

Subject(s) - biocompatibility , substrate (aquarium) , cell adhesion , sh sy5y , raman spectroscopy , materials science , bioelectronics , adhesion , nanotechnology , cell , cell culture , biophysics , viability assay , chemistry , neuroblastoma , biology , biochemistry , biosensor , ecology , genetics , physics , optics , metallurgy , composite material

A living bio-hybrid system has been successfully implemented. It is constituted by neuroblastic cells, the SH-SY5Y human neuroblastoma cells, adhering to a poly-anyline (PANI) a semiconductor polymer with memristive properties. By a multidisciplinary approach, the biocompatibility of the substrate has been analyzed and the functionality of the adhering cells has been investigated. We found that the PANI films can support the cell adhesion. Moreover, the SH-SY5Y cells were successfully differentiated into neuron-like cells for in vitro applications demonstrating that PANI can also promote cell differentiation. In order to deeply characterize the modifications of the bio-functionality induced by the cell-substrate interaction, the functional properties of the cells have been characterized by electrophysiology and Raman spectroscopy. Our results confirm that the PANI films do not strongly affect the general properties of the cells, ensuring their viability without toxic effects on their physiology. Ascribed to the adhesion process, however, a slight increase of the markers of the cell suffering has been evidenced by Raman spectroscopy and accordingly the electrophysiology shows a reduction at positive stimulations in the cells excitability

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