Open AccessThe detection and subsequent volume optimization of biological nanocrystals
Author(s) -
Joseph R. Luft,
Jennifer R. Wolfley,
Eleanor Cook Franks,
Angela Lauricella,
Ellen J. Gualtieri,
Edward H. Snell,
Rong Xiao,
J.K. Everett,
G.T. Montelione
Publication year - 2015
Publication title -
structural dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.415
H-Index - 29
ISSN - 2329-7778
DOI - 10.1063/1.4921199
Subject(s) - crystallization , nanocrystal , materials science , volume (thermodynamics) , macromolecule , excited state , biological system , throughput , nanotechnology , computer science , chemistry , physics , thermodynamics , organic chemistry , atomic physics , telecommunications , biochemistry , wireless , biology
Identifying and then optimizing initial crystallization conditions is a prerequisite formacromolecular structure determination by crystallography. Improved technologiesenable data collection on crystals that are difficult if not impossible to detect usingvisible imaging. The application of second-order nonlinear imaging of chiral crystals andultraviolet two-photon excited fluorescence detection is shown to be applicable in ahigh-throughput manner to rapidly verify the presence of nanocrystals in crystallizationscreening conditions. It is noted that the nanocrystals are rarely seen without also producingmicrocrystals from other chemical conditions. A crystal volume optimization method isdescribed and associated with a phase diagram for crystallization
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