Self assembled monolayer based liquid crystal biosensor for free cholesterol detection | Zendy

M. S. Tyagi | Zendy; Achu Chandran | Zendy; Tilak Joshi | Zendy; Jai Prakash | Zendy; Ved Varun Agrawal | Zendy; A. M. Biradar | Zendy

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Self assembled monolayer based liquid crystal biosensor for free cholesterol detection

Author(s) -

M. S. Tyagi,

Achu Chandran,

Tilak Joshi,

Jai Prakash,

Ved Varun Agrawal,

A. M. Biradar

Publication year - 2014

Publication title -

applied physics letters

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.182

H-Index - 442

eISSN - 1077-3118

pISSN - 0003-6951

DOI - 10.1063/1.4871704

Subject(s) - cholesterol oxidase , biosensor , monolayer , adsorption , self assembled monolayer , silane , chemistry , liquid crystal , scanning electron microscope , self assembly , chromatography , materials science , nanotechnology , organic chemistry , enzyme , optoelectronics , composite material

A unique cholesterol oxidase (ChOx) liquid crystal (LC) biosensor, based on the disruption of orientation in LCs, is developed for cholesterol detection. A self-assembled monolayer (SAM) of Dimethyloctadecyl[3-(trimethoxysilyl)propyl]ammonium chloride (DMOAP) and (3-Aminopropyl)trimethoxy-silane (APTMS) is prepared on a glass plate by adsorption. The enzyme (ChOx) is immobilized on SAM surface for 12 h before utilizing the film for biosensing purpose. LC based biosensing study is conducted on SAM/ChOx/LC (5CB) cells for cholesterol concentrations ranging from 10 mg/dl to 250 mg/dl. The sensing mechanism has been verified through polarizing optical microscopy, scanning electron microscopy, and spectrometric techniques.

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