Detection of organophosphorus compound based on a sol–gel silica planar waveguide doped with a green fluorescent protein and an organophosphorus hydrolase | Zendy

Yasufumi Enami | Zendy; Kenji J. Tsuchiya | Zendy; Shin-ichiro Suye | Zendy

Open Access

Detection of organophosphorus compound based on a sol–gel silica planar waveguide doped with a green fluorescent protein and an organophosphorus hydrolase

Author(s) -

Yasufumi Enami,

Kenji J. Tsuchiya,

Shin-ichiro Suye

Publication year - 2011

Publication title -

applied physics letters

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.182

H-Index - 442

eISSN - 1077-3118

pISSN - 0003-6951

DOI - 10.1063/1.3596448

Subject(s) - fluorescence , waveguide , planar , doping , green fluorescent protein , hydrolase , materials science , green light , chemistry , optoelectronics , optics , enzyme , organic chemistry , biochemistry , physics , computer graphics (images) , computer science , blue light , gene

In this letter, the authors report the real-time detection of an organophosphorus compound using a sol–gel silica planar waveguide doped with a green fluorescent protein and an organophosphorus hydrolase on a yeast-cell surface display. The waveguide was pumped at 488 nm, and it emitted green fluorescence at the far field. The green fluorescent light at 550 nm changed by 50% from the original power 1 min after application of the organophosphorus compound. The results enable the real-time detection of sarin and other biochemicals by using an in-line fiber sensor network.

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