Real-time monitoring of cell death by surface infrared spectroscopy | Zendy

Ryo-taro Yamaguchi | Zendy; Ayumi HiranoIwata | Zendy; Yasuo Kimura | Zendy; Michio Niwano | Zendy; Koichiro Miyamoto | Zendy; Hiroko Isoda | Zendy; Hitoshi Miyazaki | Zendy

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Real-time monitoring of cell death by surface infrared spectroscopy

Author(s) -

Ryo-taro Yamaguchi,

Ayumi HiranoIwata,

Yasuo Kimura,

Michio Niwano,

Koichiro Miyamoto,

Hiroko Isoda,

Hitoshi Miyazaki

Publication year - 2007

Publication title -

applied physics letters

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.182

H-Index - 442

eISSN - 1077-3118

pISSN - 0003-6951

DOI - 10.1063/1.2813013

Subject(s) - fourier transform infrared spectroscopy , infrared spectroscopy , spectroscopy , attenuated total reflection , fourier transform spectroscopy , infrared , chemistry , analytical chemistry (journal) , absorption spectroscopy , absorption (acoustics) , materials science , optics , chromatography , organic chemistry , physics , quantum mechanics , composite material

We have developed a method for real-time monitoring of the cell responses to cytotoxicants using Fourier transform infrared spectroscopy with the multiple internal reflection (MIR-FTIR) geometry. To prevent cell damages induced by measurement environments, we have constructed specialized chambers, in which temperature was maintained at (37±0.5) °C and humidified air containing 5% CO2 was supplied. We monitored cell death induced by cytotoxic surfactant Tween20 using MIR-FTIR spectroscopy. It was found that cell death can be monitored by the absorption intensity of amide II band. This result suggests that our method has a potential to be applied for real-time cytotoxicity assay

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