Escherichia coli identification and strain discrimination using nanosecond laser-induced breakdown spectroscopy | Zendy

Jonathan D. Diedrich | Zendy; Steven J. Rehse | Zendy; Sunil Palchaudhuri | Zendy

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Escherichia coli identification and strain discrimination using nanosecond laser-induced breakdown spectroscopy

Author(s) -

Jonathan D. Diedrich,

Steven J. Rehse,

Sunil Palchaudhuri

Publication year - 2007

Publication title -

applied physics letters

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.182

H-Index - 442

eISSN - 1077-3118

pISSN - 0003-6951

DOI - 10.1063/1.2723659

Subject(s) - laser induced breakdown spectroscopy , escherichia coli , strain (injury) , spectroscopy , laser , candida albicans , materials science , bacteria , analytical chemistry (journal) , chemistry , microbiology and biotechnology , biology , optics , chromatography , biochemistry , physics , genetics , anatomy , quantum mechanics , gene

Three strains of Escherichia coli, one strain of environmental mold, and one strain of Candida albicans yeast have been analyzed by laser-induced breakdown spectroscopy using nanosecond laser pulses. All microorganisms were analyzed while still alive and with no sample preparation. Nineteen atomic and ionic emission lines have been identified in the spectrum, which is dominated by calcium, magnesium, and sodium. A discriminant function analysis has been used to discriminate between the biotypes and E. coli strains. This analysis showed efficient discrimination between laser-induced breakdown spectroscopy spectra from different strains of a single bacteria species.

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