Hybrid near-field scanning optical microscopy tips for live cell measurements | Zendy

Luka K. Kapkiai | Zendy; David MooreNichols | Zendy; Jonathan Carnell | Zendy; Jeffrey R. Krogmeier | Zendy; Robert C. Dunn | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Hybrid near-field scanning optical microscopy tips for live cell measurements

Author(s) -

Luka K. Kapkiai,

David MooreNichols,

Jonathan Carnell,

Jeffrey R. Krogmeier,

Robert C. Dunn

Publication year - 2004

Publication title -

applied physics letters

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.182

H-Index - 442

eISSN - 1077-3118

pISSN - 0003-6951

DOI - 10.1063/1.1737464

Subject(s) - near field scanning optical microscope , microscopy , materials science , optical microscope , cantilever , fluorescence microscope , scanning ion conductance microscopy , fluorescence , optics , optical fiber , resolution (logic) , nanotechnology , scanning probe microscopy , optoelectronics , scanning confocal electron microscopy , scanning electron microscope , physics , composite material , artificial intelligence , computer science

We report a near-field scanning optical microscopy (NSOM) probe that enables high-resolution imaging of living cells under physiological buffered conditions. The hybrid design combines a conventional fiber optic near-field probe with a standard atomic force microscopy cantilever. Imaging of fluorescent latex spheres suspended in an acetate matrix demonstrates the subdiffraction limited fluorescence and topography capabilities of the tips. The reduced spring constant of the hybrid tip is also shown to be amenable to measurements on living cells. NSOM fluorescence and topography measurements on living human arterial smooth muscle cells under buffered conditions are demonstrated.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research