English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Background:  Proteus mirabilis  is an opportunistic pathogen, commonly associated with complicated urinary tract infections (UTIs). UTIs caused by multidrug-resistant  Proteus mirabilis  have increased worldwide. Multidrug-resistance of Gram-negative enteric bacteria is usually associated with class 1 integrons.  Purposes: To investigate the prevalence and characterize gene cassettes of class 1 integrons in multidrug-resistant  P. mirabilis    Methods: From 2006 to 2008, 314  P. mirabilis  isolates from urine were collected from a regional teaching hospital. Antimicrobial resistance of the isolates was determined by disk diffusion methods. The phenotypic confirmatory test of extended-spectrum β-lactamase (ESBL) production was performed as described in the Clinical and Laboratory Standards Institute (CLSI) guideline. The genetic organization of the class 1 integron cassettes was investigated by PCR, cloning, and sequencing of the regions surrounding these genes.  Results: Seventy-nine (25%, 79/314)  P. mirabilis  isolates were ESBL-producing and most ESBL-producing  P. mirabilis  were positive for  bla  CTX-M . Class 1 integrons were presented in 76 isolates (24.2%, 76/314), and were more frequently found in ESBL-positive (55/79, 70%) than ESBL-negative (21/235, 8.9%)  P. mirabilis  isolates. The most prevalence of the cassettes encoded resistance genes were aminoglycoside  (aac(6’)-Ib, aacA7, aadAl, aadA2,  and  aadAla),  trimethoprim  (dfrAl  and  dfrA12)  and chloramphenicol  (catB3  and  cmlA6).  The most prevalent cassette of  dfr12-orfF-aadA2  was found in 49 isolates. The cassette array  aadB-catB3-oxa10-aadA1  was first found in  P. mirabilis.  The enterobacterial repetitive intergenic consensus (ERIC)-PCR fingerprinting patterns were detected in these 76 integron positive  P. mirabilis  isolates and belonged to 8 profiles.  Conclusion: This study investigated the prevalence and characterized gene cassettes of class 1 integrons in MDR  P. mirabilis  isolates from urine samples. The frequency of gene cassettes in  P. mirabilis  were partially by clonal spread of the carriers and the results could provide information for effective antimicrobial therapy and infection control.

Genetic characteristic of class 1 integrons in proteus mirabilis isolates from urine samples