Open AccessCharacterization of microsatellite markers in the tsetse fly, Glossina pallidipes (Diptera: Glossinidae)
Author(s) -
Ouma J. O.,
Cummings M. A.,
Jones K. C.,
Krafsur E. S.
Publication year - 2003
Publication title -
molecular ecology notes
Language(s) - English
Resource type - Journals
eISSN - 1471-8286
pISSN - 1471-8278
DOI - 10.1046/j.1471-8286.2003.00480.x
Subject(s) - biology , microsatellite , tsetse fly , loss of heterozygosity , linkage disequilibrium , locus (genetics) , glossinidae , allele , genetics , zoology , ecology , haplotype , gene
Glossina pallidipes is a vector of African trypanosomiasis. Here we characterize eight new polymorphic microsatellite loci in 288 G. pallidipes sampled from 12 Kenya populations. The number of alleles per locus ranged from four to 36 with a mean of 20.5 ± 10.1. Expected single locus heterozygosities varied from 0.044 to 0.829. Heterozygosity averaged 0.616 ± 0.246. No linkage disequilibrium was found. We also report results in eight other tsetse species estimated by using the primers developed in G. pallidipes . The primers worked best in G. swynnertoni and G. austeni and worst in G. m. morsitans and G. m. submorsitans .