Open AccessIdentification and properties of microsatellite markers in tsetse flies Glossina morsitans sensu lato (Diptera: Glossinidae)
Author(s) -
Baker M. D.,
Krafsur E. S.
Publication year - 2001
Publication title -
molecular ecology notes
Language(s) - English
Resource type - Journals
eISSN - 1471-8286
pISSN - 1471-8278
DOI - 10.1046/j.1471-8278.2001.00087.x
Subject(s) - biology , microsatellite , glossina morsitans , glossinidae , allele , loss of heterozygosity , genetics , locus (genetics) , zoology , gene
Genomic libraries enriched for simple sequence repeats were constructed for Glossina morsitans morsitans , G. m. submorsitans , and G. m. centralis . Sixteen microsatellite markers were isolated from the libraries and evaluated on flies from natural G. m. morsitans populations and other Glossina species in the morsitans and palpalis species groups. The primers amplified appropriate sized DNA fragments in the morsitans and palpalis groups. In G. morsitans s.l. , eight of 12 dinucleotide repeats and four of 12 trinucleotide repeats were polymorphic. The polymorphic loci showed a mean 7.5 ± 4.8 alleles per locus and their mean heterozygosity was 55.8 ± 7.7% .