Open AccessGenetic information of three pure lines of Porphyra yezoensis (Bangiales, Rhodophyta) obtained by AFLP analysis
Author(s) -
IITSUKA OSAMU,
NAKAMURA KAYO,
OZAKI AKIYUKI,
OKAMOTO NOBUAKI,
SAGA NAOTSUNE
Publication year - 2002
Publication title -
fisheries science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.412
H-Index - 64
eISSN - 1444-2906
pISSN - 0919-9268
DOI - 10.1046/j.1444-2906.2002.00540.x
Subject(s) - amplified fragment length polymorphism , porphyra , biology , genetic analysis , genetics , population , genetic marker , primer (cosmetics) , genome , botany , gene , genetic diversity , algae , chemistry , demography , sociology , organic chemistry
: Porphyra (Bangiales, Rhodophyta), which includes several valuable marine crops, has recently received great interest as a model plant for fundamental and applied studies in marine sciences. Amplified fragment length polymorphisms (AFLPs) are a robust and efficient means for genetic mapping, linkage analysis of genetic characters for breeding and population studies in land plant genomes. To examine whether AFLPs are applicable as genetic markers in the present study, we detected AFLP markers with three pure lines in order to promote genetic analysis in Porphyra yezoensis . The following five sets of AFLP primer pairs (E‐AA, M‐CAA) (E‐AA, M‐CAC) (E‐AA, M‐CAG) (E‐AA, M‐CAT) (E‐AA, M‐CTA) were tested with template DNAs from three pure lines and they showed a total of 227 bands. This suggests that AFLP markers are promising tools for genetic analysis in Porphyra .