Open AccessGenetic diversity within and among Pinus pinaster populations: comparison between AFLP and microsatellite markers
Author(s) -
Mariette Stéphanie,
Chagné David,
Lézier Céline,
Pastuszka Patrick,
Raffin Annie,
Plomion Christophe,
Kremer Antoine
Publication year - 2001
Publication title -
heredity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.441
H-Index - 118
eISSN - 1365-2540
pISSN - 0018-067X
DOI - 10.1046/j.1365-2540.2001.00852.x
Subject(s) - biology , microsatellite , genetic diversity , amplified fragment length polymorphism , corsican , population , pinus pinaster , evolutionary biology , population genetics , zoology , genetics , ecology , allele , demography , linguistics , philosophy , sociology , gene
Twenty‐three populations of Pinus pinaster (13 Aquitaine populations and 10 Corsican populations) were analysed at three microsatellite loci and 122 AFLP loci. The aims of the study were: (i) to compare levels of within‐population and among‐population diversity assessed with both kinds of markers; (ii) to compare Aquitaine and Corsican provenances of P. pinaster; and (iii) to know if both markers gave the same information for conservation purposes. Classical population genetics statistics were estimated and the ranking of populations obtained using different markers and/or parameters were compared by computing Spearman’s rank correlations. Even though microsatellites showed a higher within‐population diversity, they showed the same level of differentiation as AFLP markers. Moreover, both markers also showed a higher genetic diversity in the Aquitaine provenance and a higher differentiation among Corsican populations. AFLPs and microsatellites gave different population diversity rankings. Consequently, the results do not support the potential population identification within each provenance for conservation purposes.