Open AccessDevelopment and characterization of DNA sequence OmyP9 associated with the sex chromosomes in rainbow trout
Author(s) -
Iturra Patricia,
Bagley Mark,
Vergara Nora,
Imbert Paula,
Medrano Juan F.
Publication year - 2001
Publication title -
heredity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.441
H-Index - 118
eISSN - 1365-2540
pISSN - 0018-067X
DOI - 10.1046/j.1365-2540.2001.00845.x
Subject(s) - biology , rainbow trout , genetics , chromosome , x chromosome , genetic marker , trout , sex linkage , dna , dna sequencing , gene , fish <actinopterygii> , fishery
This work describes the construction and characterization of a sequence characterized amplified DNA region (SCAR DNA), designated OmyP9 , that was derived from a RAPD marker associated with the sex chromosomes in rainbow trout. A Rsa I restriction fragment length polymorphism in OmyP9 identifies variants A, B and C. We found six OmyP9 variant phenotypes – A, B, C, AB, BC and ABC, in 186 individuals of seven different rainbow trout strains. The patterns of inheritance of OmyP9 in 139 fingerlings from 10 crosses of three strains of rainbow trout were studied. The males had a greater representation of the A variant (93.3%) suggesting an association with the Y chromosome. All male fingerlings analysed inherited the A variant from their male parents. These results support the hypothesis that OmyP9 is located on the sex chromosomes of rainbow trout, and that for the males studied the A variant is located on the Y chromosome in a region close to sex determinants and/or in a sector where the genetic recombination between X and Y is restricted. The present evidence also supports our previous hypothesis that OmyP9 is organized as a tandem repeated sequence in the sex chromosomes of rainbow trout. We feel that the OmyP9 Rsa I marker can be used for sex identification in crosses where it is possible to determine the phenotype of the parents.