Open AccessGenetic characterization of the silkworm Bombyx mori by simple sequence repeat (SSR)‐anchored PCR
Author(s) -
Reddy K. Damodar
Publication year - 1999
Publication title -
heredity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.441
H-Index - 118
eISSN - 1365-2540
pISSN - 0018-067X
DOI - 10.1046/j.1365-2540.1999.00607.x
Subject(s) - biology , upgma , bombyx mori , dendrogram , genetics , polymerase chain reaction , microsatellite , bombyx , mendelian inheritance , genotype , genetic diversity , gene , allele , population , demography , sociology
Thirteen diverse strains of the silkworm Bombyx mori were analysed using the simple sequence repeat anchored polymerase chain reaction (SSR‐anchored PCR) or Inter‐SSR‐PCR (ISSR‐PCR). A set of four 5′‐anchored and two 3′‐anchored repeat primers amplified a total of 239 bands out of which 184 (77%) were polymorphic. The 5′‐anchored primers revealed more distinct polymorphic markers than the 3′‐anchored primers and the ISSR‐PCR method showed greater variability than RAPDs. The strain‐specific pattern was shown to be inherited and segregated in a Mendelian fashion. A dendrogram constructed using the UPGMA method revealed two distinct groups, one comprising nondiapausing and one comprising diapausing strains. These results suggest that the ISSR‐PCR method is potentially useful for genetic fingerprinting of silkworm genotypes and as a mapping tool in the silkworm.