Open AccessGenetics of esterase‐mediated insecticide resistance in the aphid Schizaphis graminum
Author(s) -
S. Dean Rider,
Gerald E. Wilde,
Srinivas Kambhampati
Publication year - 1998
Publication title -
heredity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.441
H-Index - 118
eISSN - 1365-2540
pISSN - 0018-067X
DOI - 10.1046/j.1365-2540.1998.00375.x
Subject(s) - biology , aphid , esterase , genetics , aphididae , gene , clone (java method) , restriction fragment length polymorphism , botany , homoptera , pest analysis , enzyme , genotype , biochemistry
The genetics of organophosphate resistance, including the pattern of inheritance and the underlying genetic mechanism, were investigated in the aphid Schizaphis graminum (the greenbug). Resistant greenbugs with pattern 1 (R1) and pattern 2 (R2) esterase and susceptible (S) greenbugs were induced into the sexual cycle and crossed. Each resistance‐associated esterase pattern in the greenbug was inherited in a Mendelian fashion as a single gene. An aphid clone that displayed both resistance‐associated esterase patterns was produced in the F 1 generation of a cross between R2 and R1 insects, and was designated pattern 3 (R3). DNA from different R1 aphids and the R3 aphid clone displayed a restriction fragment pattern different from that of R2 and S clones, and about a twofold increase in hybridization compared with S aphids. DNA from R2 clones showed no restriction fragment differences or increased hybridization signal compared to S insects. These findings suggest that the mechanism behind the R1 esterase activity may be gene amplification, but that the R2 elevated esterase activity may result from a different genetic mechanism.