Open AccessProduction of nitrite by dengue virus‐induced cytotoxic factor
Author(s) -
MISRA A.,
MUKERJEE RUMA,
CHATURVEDI U. C.
Publication year - 1996
Publication title -
clinical & experimental immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.329
H-Index - 135
eISSN - 1365-2249
pISSN - 0009-9104
DOI - 10.1046/j.1365-2249.1996.34743.x
Subject(s) - dengue fever , dengue virus , cytotoxic t cell , immunology , virology , nitrite , biology , virus , medicine , ecology , genetics , in vitro , nitrate
Dengue type 2 virus (DV) infection induces production of a cytokine, the cytotoxic factor (CF) in the spleen of mice. The present study was undertaken to investigate the production of nitrite (NO − 2 ) by the spleen cells of mice in vitro and in vivo following inoculation of DV or CF. Maximum NO − 2 production occurred at 45 min after inoculation of 5 μg CF, both in vitro and in vivo . The NO − 2 was produced by macrophages and T cells and not by B cells. Pretreatment of CF with anti‐CF antisera inhibited production of NO − 2 . DV‐stimulated spleen cell culture supernatants showed peak production of CF and NO − 2 at 72 h. In DV‐infected mouse spleen, maximum NO − 2 production occurred at 8–11 days post‐infection, which correlated with peak cytotoxic activity in the spleen. Pretreatment of spleen cells with N G ‐monomethyl l ‐arginine (NMMA) inhibited NO − 2 production. NO − 2 production was abrogated in a dose‐dependent manner by treatment of spleen cells with Ca 2+ channel blocking drug, Nifedipine. The findings demonstrate that DV‐induced CF induces production of NO − 2 in spleen cells, probably in a Ca 2+ ‐dependent manner, and may be a mechanism of target cell killing.