Open AccessRed blood cells inhibit tumour cell adhesion to the peritoneum
Author(s) -
Rossen M. E. E. van,
Stoop M. P. O.,
Hofland L. J.,
Koetsveld P. M. van,
Bonthuis F.,
Jeekel J.,
Marquet R. L.,
Eijck C. H. J. van
Publication year - 1999
Publication title -
british journal of surgery
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.202
H-Index - 201
eISSN - 1365-2168
pISSN - 0007-1323
DOI - 10.1046/j.1365-2168.1999.01050.x
Subject(s) - medicine , in vivo , adhesion , cell adhesion , peritoneum , red blood cell , peritoneal cavity , in vitro , cell , blood cell , pathology , immunology , cancer research , biology , surgery , chemistry , biochemistry , microbiology and biotechnology , organic chemistry
Background: Perioperative blood transfusion has been associated with increased tumour recurrence and poor prognosis in colorectal cancer. Blood loss in the peritoneal cavity might be a tumour‐promoting factor for local recurrence. The aim of this study was to investigate whether blood in the peritoneal cavity affects local tumour recurrence. Methods: In an established in vivo rat model the effect of 1·5 ml syngeneic whole blood on tumour cell adhesion and tumour growth was investigated. In the same model the effect of 1·5 ml pure red blood cell (RBC) concentrate and 1·5 ml RBC‐derived substances on tumour cell adhesion was studied. In an established in vitro model the effect of increasing numbers of RBCs (0–250 × 10 6 ) on tumour cell adhesion and tumour growth was assessed. Results: Both the presence of blood and RBC concentrate in the peritoneal cavity prevented tumour cell adhesion in vivo (overall P ≤ 0·001 and P ≤ 0·05 respectively), rather than promoting adherence. RBC concentrate and RBC‐derived substances had a comparable inhibitory effect on tumour cell adhesion. In in vitro studies RBCs inhibited tumour cell adhesion but not tumour growth. Conclusion: RBC‐derived factors prevent tumour cell adhesion to the peritoneum, and consequently tumour recurrence. © 1999 British Journal of Surgery Society Ltd