Open AccessInhibition of platelet‐activating factor, intercellular adhesion molecule 1 and platelet endothelial cell adhesion molecule 1 reduces experimental pancreatitis‐associated gut endothelial barrier dysfunction
Author(s) -
Wang X.,
Sun Z.,
Börjesson A.,
Andersson R.
Publication year - 1999
Publication title -
british journal of surgery
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.202
H-Index - 201
eISSN - 1365-2168
pISSN - 0007-1323
DOI - 10.1046/j.1365-2168.1999.01028.x
Subject(s) - intercellular adhesion molecule 1 , endothelial dysfunction , medicine , endothelial stem cell , cell adhesion molecule , platelet activating factor , icam 1 , endothelium , intercellular adhesion molecule , inflammation , acute pancreatitis , platelet , immunology , cell adhesion , adhesion , biology , chemistry , biochemistry , in vitro , organic chemistry
Background: Endothelial barrier dysfunction is a critical link in the development of tissue injury and organ dysfunction, via upregulation and exposure of adhesion molecules, intercellular signals and leucocyte–endothelial cell interactions. Inhibitors of inflammatory mediators and receptors have been suggested as a means of downregulating the cascade of both local and systemic inflammation. Methods: The potential therapeutic inhibition of platelet‐activating factor (PAF), intercellular adhesion molecule (ICAM) 1 and platelet endothelial cell adhesion molecule (PECAM) 1 was investigated in pancreatitis‐associated gut endothelial dysfunction in rats, by treatment with a PAF antagonist (lexipafant, BB‐882) and monoclonal antibodies against rat ICAM‐1 (anti‐ICAM1‐Mb) and PECAM (anti‐PECAM1‐Mb). Alterations in gut endothelial barrier dysfunction and leucocyte recruitment, and systemic levels of interleukins were evaluated. Results: Plasma exudation measured by the albumin leakage index and tissue leucocyte recruitment in the distal small intestine and colon increased significantly 12 h after induction of pancreatitis and treatment with saline. These alterations were to varying degrees counteracted by treatment with lexipafant, anti‐ICAM1‐Mb or anti‐PECAM1‐Mb. Alterations in levels of interleukin (IL) 1 paralleled the changes in gut endothelial barrier dysfunction and leucocyte trapping. Conclusion: Treatment with lexipafant and monoclonal antibodies against ICAM‐1 or PECAM‐1 reduced the severity of pancreatitis‐associated gut endothelial dysfunction, and decreased systemic concentrations of IL‐1 and local leucocyte recruitment. © 1999 British Journal of Surgery Society Ltd