Open AccessMatrix metalloproteinase 9 level predicts optimal collagen deposition during early wound repair in humans
Author(s) -
Ågren M. S.,
Jorgensen I. N.,
Andersen M.,
Viljanto J.,
Gottrup P.
Publication year - 1998
Publication title -
british journal of surgery
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.202
H-Index - 201
eISSN - 1365-2168
pISSN - 0007-1323
DOI - 10.1046/j.1365-2168.1998.00556.x
Subject(s) - hydroxyproline , matrix metalloproteinase , medicine , wound healing , matrix (chemical analysis) , hernia , hernia repair , metalloproteinase , surgery , pathology , chromatography , chemistry
Background This study examined two matrix metalloproteinases (MMPs) and their association with deposition of collagen during wound repair. Methods In 15 hernia wounds, wound fluid was collected using two Cellstick devices, which were implanted adjacent to two expanded polytetrafluoroethylene (ePTFE) tubes. MMP‐2 and MMP‐9 levels were analysed 24 and 48 h after operation using specific enzyme‐linked immunosorbent assays, and collagen deposition in the ePTFE tubes was measured 10 days after operation as hydroxyproline. Results The levels of both MMPs in wound fluid were increased compared with those of control serum, although MMP‐9 decreased ( P < 0·01) in the wounds 24–48 h after operation. The MMP‐9 level at 24 h correlated inversely and specifically to deposited collagen measured as hydroxyproline ( r S = −0·80, P < 0·01). Conclusion These results indicate that the level of MMP‐9 in the early inflammatory phase can predict the amount of collagen deposited later in the wound healing process. © 1998 British Journal of Surgery Society Ltd