Cryo-soft X-ray tomography: using soft X-rays to explore the ultrastructure of whole cells | Zendy

Maria Harkiolaki | Zendy; Michele C. Darrow | Zendy; Matthew C. Spink | Zendy; Ewelina Kosior | Zendy; Kyle C. Dent | Zendy; Elizabeth Duke | Zendy

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Cryo-soft X-ray tomography: using soft X-rays to explore the ultrastructure of whole cells

Author(s) -

Maria Harkiolaki,

Michele C. Darrow,

Matthew C. Spink,

Ewelina Kosior,

Kyle C. Dent,

Elizabeth Duke

Publication year - 2018

Publication title -

emerging topics in life sciences

Language(s) - English

Resource type - Journals

eISSN - 2397-8562

pISSN - 2397-8554

DOI - 10.1042/etls20170086

Subject(s) - ultrastructure , tomography , microscopy , resolution (logic) , biomedical engineering , sample (material) , biological specimen , materials science , computer science , optics , chemistry , pathology , physics , medicine , artificial intelligence , chromatography

Cryo-soft X-ray tomography is an imaging technique that addresses the need for mesoscale imaging of cellular ultrastructure of relatively thick samples without the need for staining or chemical modification. It allows the imaging of cellular ultrastructure to a resolution of 25-40 nm and can be used in correlation with other imaging modalities, such as electron tomography and fluorescence microscopy, to further enhance the information content derived from biological samples. An overview of the technique, discussion of sample suitability and information about sample preparation, data collection and data analysis is presented here. Recent developments and future outlook are also discussed.

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