Open AccessEffect of 60 Hz electromagnetic fields on the activity of hsp70 promoter: an in vivo study
Author(s) -
Fuente RodríguezDe Abraham O.,
AlcocerGonzález Juan M.,
HerediaRojas J. Antonio,
RodríguezPadilla Cristina,
RodríguezFlores Laura E.,
SantoyoStephano Martha A.,
CastañedaGarza Esperanza,
TamëzGuerra Reyes S.
Publication year - 2012
Publication title -
cell biology international reports
Language(s) - English
Resource type - Journals
ISSN - 2041-5346
DOI - 10.1042/cbr20110010
Subject(s) - luciferase , hsp70 , plasmid , in vivo , gene expression , gene , reporter gene , heat shock protein , biology , microbiology and biotechnology , chemistry , transfection , genetics
Exposure to EMFs (electromagnetic fields) results in a number of important biological changes, including modification of genetic expression. We have investigated the effect of 60 Hz sinusoidal EMFs at a magnetic flux density of 80 μT on the expression of the luciferase gene contained in a plasmid labelled as pEMF (EMF plasmid). This gene construct contains the specific sequences for the induction of hsp70 (heat‐shock protein 70) expression by EMFs, as well as the reporter for the luciferase gene. The pEMF vector was electrotransferred into quadriceps muscles of BALB/c mice that were later exposed to EMFs. Increased luciferase expression was observed in mice exposed to EMFs 2 h daily for 7 days compared with controls ( P <0.05). These data along with other reports in the literature suggest that EMFs can have far‐reaching effects on the genome.
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