Resistance characteristics of CTX-M typeShigella flexneriin China | Zendy

Feng-zhi Bian | Zendy; Mingxiao Yao | Zendy; Hongmei Fu | Zendy; Guangying Yuan | Zendy; Shuzhi Wu | Zendy; Sun Yu-guo | Zendy

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Resistance characteristics of CTX-M typeShigella flexneriin China

Author(s) -

Feng-zhi Bian,

Mingxiao Yao,

Hongmei Fu,

Guangying Yuan,

Shuzhi Wu,

Sun Yu-guo

Publication year - 2019

Publication title -

bioscience reports

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.938

H-Index - 77

eISSN - 1573-4935

pISSN - 0144-8463

DOI - 10.1042/bsr20191741

Subject(s) - shigella flexneri , microbiology and biotechnology , cefotaxime , cefoxitin , ceftazidime , biology , ciprofloxacin , cefepime , imipenem , ampicillin , genotype , shigella , tetracycline , drug resistance , sulfamethoxazole , virology , antibiotic resistance , antibiotics , salmonella , bacteria , gene , escherichia coli , genetics , pseudomonas aeruginosa , staphylococcus aureus

The present study was to identify the drug resistance, resistance mechanism and the extended-spectrum β-lactamase (ESBLs) genotypes of Shigella flexneri ( S. flexneri ) in Jinan. Susceptibility tests were performed by MIC-determination. The genotypes of β-lactamase were identified using PCR and DNA sequencing. The resistance transfer ability of the ESBL-producing strains was examined by conjugation tests. A total of 105 S. flexneri isolates were collected, and 34 (32.4%) were ESBL-producing isolates. All ESBL-producing isolates were susceptible to cefoxitin and imipenem, and 35.3% isolates were resistant to ciprofloxacin. ESBL-producing isolates showed high level resistant to ampicillin (100%), cefotaxime (100%), tetracycline (100%), chloramphenicol (100%), trimethoprim/sulfamethoxazole (100%), ceftazidime (73.5%) and cefepime (73.5%). Three types of β-lactamase genes ( bla TEM, bla OXA and bla CTX-M) were identified in all ESBL-producing isolates, and the genotypes were confirmed as bla TEM-1 (23/34), bla OXA-30 (34/34), bla CTX-M-14 (9/34) and bla CTX-M-15 (25/34) by sequencing. In conclusion, the Shigella strains isolated in Jinan are cross-resistant and multi-drug resistant. The main genotypes of ESBLs are CTX-M-14 and CTX-M-15.

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