Placenta-specific 9, a putative secretory protein, induces G2/M arrest and inhibits the proliferation of human embryonic hepatic cells | Zendy

Cong Ouyang | Zendy; Yi-Zhi Pu | Zendy; Xu-Hui Qin | Zendy; Jinhua Shen | Zendy; Qinghua Liu | Zendy; Liqun Ma | Zendy; Lu Xue | Zendy

Open Access

Placenta-specific 9, a putative secretory protein, induces G2/M arrest and inhibits the proliferation of human embryonic hepatic cells

Author(s) -

Cong Ouyang,

Yi-Zhi Pu,

Xu-Hui Qin,

Jinhua Shen,

Qinghua Liu,

Liqun Ma,

Lu Xue

Publication year - 2018

Publication title -

bioscience reports

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.938

H-Index - 77

eISSN - 1573-4935

pISSN - 0144-8463

DOI - 10.1042/bsr20180820

Subject(s) - microbiology and biotechnology , golgi apparatus , endoplasmic reticulum , biology , flow cytometry , secretory pathway , cell growth , cell cycle , cytoplasm , embryonic stem cell , embryogenesis , secretory protein , context (archaeology) , cell , secretion , embryo , biochemistry , gene , paleontology

Background: Placenta-specific 9 ( Plac9 ) is a putative secreted protein that was first discovered in the context of embryogenesis. The expression pattern of Plac9 during embryogenesis, together with the results of recent reports, suggest that Plac9 may play a role in the liver development. The present study was conducted to investigate the secretory characteristics of Plac9 and its potential role in liver cell physiology. Methods: Immunofluorescence was employed to identify the subcellular distribution of Plac9 Cellular proliferative activity was analyzed by MTT assay and cell colony formation. The cell cycle distribution of Plac9 was analyzed by flow cytometry, and a functional analysis was performed using L02 cells following their stable infection with a lentivirus over-expressing Plac9 Results: Plac9 is a novel protein that is localized to the cytoplasm and may be secreted through the classic endoplasmic reticulum-Golgi route. The overexpression of Plac9 inhibits cell growth and induces G2/M phase arrest. Conclusion: Our findings reveal a novel role for Plac9 in regulating cell growth.

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