An accurate and cost-effective alternative method for measuring cell migration with the circular wound closure assay | Zendy

Michael L. De Ieso | Zendy; Jinxin V. Pei | Zendy

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An accurate and cost-effective alternative method for measuring cell migration with the circular wound closure assay

Author(s) -

Michael L. De Ieso,

Jinxin V. Pei

Publication year - 2018

Publication title -

bioscience reports

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.938

H-Index - 77

eISSN - 1573-4935

pISSN - 0144-8463

DOI - 10.1042/bsr20180698

Subject(s) - wound closure , scratch , reproducibility , biological system , objectivity (philosophy) , biomedical engineering , cell migration , computer science , wound healing , chemistry , cell , biology , chromatography , surgery , medicine , biochemistry , philosophy , epistemology , operating system

Cell migration is important in many physiological and pathological processes. Mechanisms of two-dimensional cell migration have been investigated most commonly by evaluating rates of cell migration into linearly scratched zones on the surfaces of culture plates. Here, we present a detailed description of a simple adaptation for the well-known and popular wound closure assay, using a circular wound instead of a straight line. This method demonstrates improved precision, reproducibility, and sampling objectivity for measurements of wound sizes as compared with classic scratch assays, enabling more accurate calculations of migration rate. The added benefits of the method are simplicity and low cost as compared with commercially available assays for generating circular wounds.

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