Low expression of miR-30a-5p induced the proliferation and invasion of oral cancer via promoting the expression of FAP | Zendy

Peng Ruan | Zendy; Zezhang Tao | Zendy; Aili Tan | Zendy

Open Access

Low expression of miR-30a-5p induced the proliferation and invasion of oral cancer via promoting the expression of FAP

Author(s) -

Peng Ruan,

Zezhang Tao,

Aili Tan

Publication year - 2017

Publication title -

bioscience reports

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.938

H-Index - 77

eISSN - 1573-4935

pISSN - 0144-8463

DOI - 10.1042/bsr20171027

Subject(s) - microrna , transfection , cancer research , cancer , cancer cell , luciferase , cell growth , chemistry , biology , microbiology and biotechnology , cell culture , gene , biochemistry , genetics

The study aimed at investigating the effects of miR-30a-5p on the biological functions of oral cancer cells and figuring out the potential mechanism. We first verified the low expression of miR-30a-5p and high expression of FAP ( Homo sapiens fibroblast activation protein α) in oral cancerous tissues and their negative correlation. Then, the target relationship between miR-30a-5p and FAP was validated by dual luciferase reporter assay and biotin-coupled miRNA pulldown assay. After transfection in Tca-8113 cells and SCC-15 cells, MTT, colony formation, Transwell, and wound healing assays were performed to investigate how miR-30a-5p and FAP adjusted propagation, invasiveness, and migration, respectively. Mounting evidence supported that miR-30a-5p directly targetted FAP and suppressed its expression in oral cavity cancer cells (OSCCs). By suppressing FAP expression, miR-30a-5p significantly inhibited cell propagation, migration, and invasion. Therefore, miR-30a-5p might be a new therapeutic target for oral cancer treatment.

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