Open AccessRBFOX3 regulates Claudin-1 expression in human lung tissue via attenuation of proteasomal degradation
Author(s) -
YongEun Kim,
Sunkyung Choi,
Jong Ok Kim,
Kee K. Kim
Publication year - 2017
Publication title -
bioscience reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.938
H-Index - 77
eISSN - 1573-4935
pISSN - 0144-8463
DOI - 10.1042/bsr20160623
Subject(s) - claudin , cycloheximide , messenger rna , microbiology and biotechnology , immunostaining , alternative splicing , biology , rna binding protein , rna splicing , ubiquitin , cytoplasm , regulator , rna , chemistry , tight junction , protein biosynthesis , gene , immunohistochemistry , biochemistry , immunology
RBFOX3, a nuclear RNA-binding protein, is well known as a regulator of alternative pre-mRNA splicing during neuronal development. However, other functions of RBFOX3 are poorly understood. Here, we investigated the function of RBFOX3 in the cytoplasm with respect to regulation of Claudin-1 expression. In human lung tissue, Claudin-1 is higher in RBFOX3-positive cells than in RBFOX3-negative cells. Immunostaining and mRNA quantification revealed that protein levels, but not mRNA levels, of Claudin-1 are increased by RBFOX3. In addition, cycloheximide treatment of human lung cancer cells revealed that RBFOX3 increases the stability of Claudin-1 through attenuation of its ubiquitination. Our study provides insights into the molecular mechanisms by which RBFOX3 regulates Claudin-1 expression in human lung tissue.
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