Smooth muscle titin forms in vitro amyloid aggregates | Zendy

А. Г. Бобылев | Zendy; Oxana V. Galzitskaya | Zendy; Р. С. Фадеев | Zendy; L. G. Bobyleva | Zendy; D. A. Yurshenas | Zendy; Nikolay V. Molochkov | Zendy; Nikita V. Dovidchenko | Zendy; Olga M. Selivanova | Zendy; Nikita V. Penkov | Zendy; Z. A. Podlubnaya | Zendy; I. M. Vikhlyantsev | Zendy

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Smooth muscle titin forms in vitro amyloid aggregates

Author(s) -

А. Г. Бобылев,

Oxana V. Galzitskaya,

Р. С. Фадеев,

L. G. Bobyleva,

D. A. Yurshenas,

Nikolay V. Molochkov,

Nikita V. Dovidchenko,

Olga M. Selivanova,

Nikita V. Penkov,

Z. A. Podlubnaya,

I. M. Vikhlyantsev

Publication year - 2016

Publication title -

bioscience reports

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.938

H-Index - 77

eISSN - 1573-4935

pISSN - 0144-8463

DOI - 10.1042/bsr20160066

Subject(s) - titin , thioflavin , amyloid (mycology) , biophysics , amyloidosis , chemistry , protein aggregation , in vitro , obscurin , fibril , confocal microscopy , actin , biochemistry , microbiology and biotechnology , myocyte , sarcomere , pathology , biology , alzheimer's disease , medicine , inorganic chemistry , disease

Amyloids are insoluble fibrous protein aggregates, and their accumulation is associated with amyloidosis and many neurodegenerative diseases, including Alzheimer's disease. In the present study, we report that smooth muscle titin (SMT; 500 kDa) from chicken gizzard forms amyloid aggregates in vitro This conclusion is supported by EM data, fluorescence analysis using thioflavin T (ThT), Congo red (CR) spectroscopy and X-ray diffraction. Our dynamic light scattering (DLS) data show that titin forms in vitro amyloid aggregates with a hydrodynamic radius (Rh) of approximately 700-4500 nm. The initial titin aggregates with Rh approximately 700 nm were observed beyond first 20 min its aggregation that shows a high rate of amyloid formation by this protein. We also showed using confocal microscopy the cytotoxic effect of SMT amyloid aggregates on smooth muscle cells from bovine aorta. This effect involves the disorganization of the actin cytoskeleton and result is cell damage. Cumulatively, our results indicate that titin may be involved in generation of amyloidosis in smooth muscles.

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