Unusual organization, complexity and redundancy at the Escherichia coli hcp-hcr operon promoter | Zendy

David L. Chismon | Zendy; Douglas F. Browning | Zendy; Gregory K. Farrant | Zendy; Stephen Busby | Zendy

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Unusual organization, complexity and redundancy at the Escherichia coli hcp-hcr operon promoter

Author(s) -

David L. Chismon,

Douglas F. Browning,

Gregory K. Farrant,

Stephen Busby

Publication year - 2010

Publication title -

biochemical journal

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.706

H-Index - 265

eISSN - 1470-8728

pISSN - 0264-6021

DOI - 10.1042/bj20100623

Subject(s) - operon , escherichia coli , transcription (linguistics) , l arabinose operon , biology , dna , random hexamer , transcription factor , promoter , binding site , genetics , chemistry , microbiology and biotechnology , biochemistry , gene , gene expression , linguistics , philosophy

Expression from the Escherichia coli hcp-hcr operon promoter is optimally induced during anaerobic conditions in the presence of nitrite. This expression depends on transcription activation by FNR (fumarate and nitrate reduction regulator), which binds to a target centred at position -72.5 upstream of the transcript start site. Mutational analysis was exploited to identify the corresponding -10 and -35 hexamer elements. A DNA site for NarL and NarP, located at position -104.5, plays only a minor role, whereas NsrR binding to a DNA target centred at position +6 plays a major role in induction of the hcp-hcr operon promoter. Electrophoretic mobility-shift assays show that NsrR binds to this target. The consequences of this for the kinetics of induction of the hcp-hcr operon are discussed.

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