Nucleosomes represent a physical barrier for cleavage activity of DNA topoisomerase I in vivo | Zendy

Francesca Felice | Zendy; Francesco Chiani | Zendy; Giorgio Camilloni | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Nucleosomes represent a physical barrier for cleavage activity of DNA topoisomerase I in vivo

Author(s) -

Francesca Felice,

Francesco Chiani,

Giorgio Camilloni

Publication year - 2008

Publication title -

biochemical journal

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.706

H-Index - 265

eISSN - 1470-8728

pISSN - 0264-6021

DOI - 10.1042/bj20070893

Subject(s) - topoisomerase , dna supercoil , dna , histone , dna replication , histone octamer , biology , cleavage (geology) , nucleosome , eukaryotic dna replication , saccharomyces cerevisiae , transcription (linguistics) , biochemistry , microbiology and biotechnology , chemistry , genetics , gene , paleontology , linguistics , philosophy , fracture (geology)

DNA topoisomerase I together with the other cellular DNA topoisomerases releases the torsional stress from DNA caused by processes such as replication, transcription and recombination. Despite the well-defined knowledge of its mechanism of action, DNA topoisomerase I in vivo activity has been only partially characterized. In fact the basic question concerning the capability of the enzyme to cleave and rejoin DNA wrapped around a histone octamer remains still unanswered. By studying both in vivo and in vitro the cleavage activity of DNA topoisomerase I in the presence of camptothecin on a repeated trinucleotide sequence, (TTA)(35), lying in chromosome XIII of Saccharomyces cerevisiae, we can conclude that nucleosomes represent a physical barrier for the enzyme activity.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research