A beginner’s guide to mass spectrometry–based proteomics | Zendy

Ankit Sinha | Zendy; Matthias Mann | Zendy

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A beginner’s guide to mass spectrometry–based proteomics

Author(s) -

Ankit Sinha,

Matthias Mann

Publication year - 2020

Publication title -

the biochemist

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.126

H-Index - 7

eISSN - 1740-1194

pISSN - 0954-982X

DOI - 10.1042/bio20200057

Subject(s) - proteomics , mass spectrometry , grasp , quantitative proteomics , profiling (computer programming) , chemistry , computer science , instrumentation (computer programming) , sample preparation , data science , computational biology , chromatography , biology , biochemistry , software engineering , gene , operating system

Mass spectrometry (MS)-based proteomics is the most comprehensive approach for the quantitative profiling of proteins, their interactions and modifications. It is a challenging topic as a firm grasp requires expertise in biochemistry for sample preparation, analytical chemistry for instrumentation and computational biology for data analysis. In this short guide, we highlight the various components of a mass spectrometer, the sample preparation process for conversion of proteins into peptides, and quantification and analysis strategies. The advancing technology of MS-based proteomics now opens up opportunities in clinical applications and single-cell analysis.

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