Beginner’s guide to producing super-resolved images on a widefield fluorescence microscope | Zendy

Ilijana Vojnovic | Zendy; Ulrike Endesfelder | Zendy

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Beginner’s guide to producing super-resolved images on a widefield fluorescence microscope

Author(s) -

Ilijana Vojnovic,

Ulrike Endesfelder

Publication year - 2020

Publication title -

the biochemist

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.126

H-Index - 7

eISSN - 1740-1194

pISSN - 0954-982X

DOI - 10.1042/bio20200045

Subject(s) - microscope , microscopy , fluorescence microscope , optics , computer science , visualization , fluorescence , super resolution microscopy , nanometre , photoactivated localization microscopy , nanotechnology , materials science , physics , artificial intelligence

The development of superresolution microscopy techniques, which are able to achieve resolutions in the nanometre range and as such allow the visualization of subcellular structures and dynamics, has considerably expanded the possibilities of fluorescence microscopy in the life sciences. While a majority of these techniques require highly specialized hardware, singlemolecule localization microscopy (SMLM) can be implemented on conventional widefield fluorescence microscopes. Here, we describe what technical upgrades are necessary and discuss some of the difficulties that can be encountered during sample preparation and imaging. Ilijana Vojnovic and Ulrike Endesfelder (Max Planck Institute for Terrestrial Microbiology and LOEWE Center for Synthetic Microbiology (SYNMIKRO), Germany) Beginner's Guide

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