Studying live embryos in 4D: Selective plane illumination microscopy: a new technique for high-resolution four-dimensional imaging | Zendy

Jan Huisken | Zendy

Open Access

Studying live embryos in 4D: Selective plane illumination microscopy: a new technique for high-resolution four-dimensional imaging

Author(s) -

Jan Huisken

Publication year - 2005

Publication title -

the biochemist

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.126

H-Index - 7

eISSN - 1740-1194

pISSN - 0954-982X

DOI - 10.1042/bio02705013

Subject(s) - light sheet fluorescence microscopy , optical sectioning , microscopy , confocal microscopy , optics , bright field microscopy , materials science , confocal , scanning confocal electron microscopy , fluorescence microscope , optical microscope , microscope , resolution (logic) , penetration depth , fluorescence , scanning electron microscope , physics , artificial intelligence , computer science

Selective plane illumination microscopy (SPIM) offers an alternative way of optically sectioning the sample in fluorescence microscopy. By illuminating the sample with a sheet of light, a sectioning effect can be obtained that is similar to that in confocal microscopy. However, in combination with widefield detection, such an approach has several advantages over confocal scanning microscopy. SPIM offers reduced fluorophore1 bleaching, fast, highly efficient image recording, and high depth penetration, especially when multiple views are combined. SPIM performs especially well in large samples such as fish or fly embryos, which can be observed live for several days. The principle is universal and can also be applied to micrometre-sized samples.

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