The worm's turn: Genome-wide RNAi in Caenorhabditis elegans | Zendy

Gino Poulin | Zendy; David P. Welchman | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

The worm's turn: Genome-wide RNAi in Caenorhabditis elegans

Author(s) -

Gino Poulin,

David P. Welchman

Publication year - 2004

Publication title -

the biochemist

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.126

H-Index - 7

eISSN - 1740-1194

pISSN - 0954-982X

DOI - 10.1042/bio02605025

Subject(s) - rna interference , caenorhabditis elegans , biology , gene silencing , gene , genome , computational biology , genetics , model organism , rna

RNAi-mediated silencing is now being used in multiple species, from plants to humans, to identify gene functions. This new technology is fully exploited in Caenorhabditis elegans, since an RNAi feeding library that has the potential to inactivate 86% of the predicted genes of the C. elegans genome is publicly available. This tool made systematic screening of genes involved in biological processes of interest easier, but there are some pitfalls: specificity, reproducibility and hypomorphic effects. However, as with any other technology, RNAi is getting better as time goes on. Once these caveats are taken into account, RNAi-mediated silencing is still a very powerful approach to identify new gene functions and to dissect complex pathways.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research